Fermentation Method

Correction of pH and dissolved oxygen electrodes.

Tank sterilization. Medium into the tank as needed, as required to seal the tank add large autoclaves and sterilization (115 degrees, 30 minutes).

Tank cooled, place it in the fermentation stage, install good; open cooling water, power up the pump and start connecting ventilation pipe ventilation, adjust the air inlet knob ventilation suitable power on the fermentation tanks, settings, such as temperature, pH, agitation speed, 640r/min power turn 30min, dissolved oxygen electrode setting is 100.

Temperature stability, parameters are correct, the rocking good seed access, start fermentation time, and began recording various parameters.

Cleaning after completion of fermentation tank and electrodes, electrodes inserted in flask 4 m KCL for later use.